KINASE

In chemistry and biochemistry, a 'kinase', alternatively known as a 'phosphotransferase', is a type of enzyme that transfers phosphate groups from high-energy donor molecules, such as ATP, to specific target molecules (substrates); the process is termed ''phosphorylation'' (An enzyme that ''removes'' phosphate groups from targets is known as a phosphatase.)

Contents

Functions of phosphorylation

Types

See also

Functions of phosphorylation

In some settings, the purpose of phosphorylation is to "activate" or "energize" a molecule, increasing its energy so it is able to participate in a subsequent reaction with a negative free-energy change. All kinases require a divalent metal ion such as Mg²⁺ or Mn²⁺ to be present, which stabilizes the high-energy bonds of the donor molecule (usually ATP or ATP derivative) and allows phosphorylation to occur.
In other instances, phosphorylation of a protein substrate can inhibit its activity (as when AKT phosphorylates the enzyme GSK-3). One common mechanism for phosphorylation-mediated enzyme inhibition was demonstrated in the tyrosine kinase called "src" (pronounced "sarc", see: Src (gene)). When src is phosphorylated on a particular tyrosine, it folds on itself, and thus masks its own kinase domain, and is thus shut "off".
In still other instances, phosphorylation of a protein causes it to be bound to other proteins which have "recognition domains" for a phosphorylated tyrosine, serine, or threonine motif. As a result of binding a particular protein, a distinct signaling system may be activated or inhibited.
In the late 1990s it was recognized that phosphorylation of some proteins causes them to be degraded by the ATP-dependent ubiquitin/proteasome pathway. These target proteins become substrates for particular E3 ubiquitin ligases only when they are phosphorylated.

Types

The largest group of kinases are protein kinases, which act on and modify the activity of specific proteins. These are used extensively to transmit signals and control complex processes in cells. Up to 518 different kinases have been identified in humans. Their enormous diversity and role in signalling makes them attractive targets for drug design.
Various other kinases act on small molecules (lipids, carbohydrates, amino acids, nucleotides, and more), either for signaling or to prime them for biochemical reactions in metabolism. These are named after their substrates.

See also

★ Signal transduction

★ G protein-coupled receptor